Abstract

Structural DNA nanotechnology has emerged as a powerful approach for the manipulation of lipid membranes on model systems as well as live cells. This approach enables the creation of nanoscale structures and machines that mimic the function of molecular machines and enable molecular inquiry with increasing capability. Because these nanostructures are anisotropic, both shape and decoration modulate how they are able to interact with cell membranes. To explore and realize the potential for synthetic biological platforms as sensors and actuators we need to (1) demonstrate compatibility with a range of cell types, (2) expand the capabilities of our design tools and (3) address the stability of these systems in terms of degradation. Accordingly, in this seminar I will present a variety of nanostructural approaches for labeling mechanosensitive cells, assessing glyococalyx health, and modulating cellular biomechanics. I will also discuss how software design limitations can be addressed using emerging automated design approaches. And finally I will introduce peptide nucleic acid (PNA) nanomaterials that may provide improved nanosystem stability in vitro and in vivo. Taken together, these advances point to a next generation synthetic biological sensors and actuators that will enable minimally destructive interfaces, novel architectures and building materials that enable enhanced device performance and enzymatic resistance.