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LMB Seminar - Fast and slow pathways of ribosomal subunit association

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We have studied Mg+ enforced ribosomal subunit association by time-resolved cryo-EM, and found several intermediates states that indicate the existence of multiple pathways. One of these is very rapid (~10 ms), the others are in the range of hundreds of ms. The results can be rationalized on the basis of electrostatic interactions and bridge formations between subunits incubated with Mg+ ions.

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