Abstract

This event is a discussion forum, focused on comparing and contrasting available techniques that measure cell mediated immunity. The study of cell mediated immunity requires the identification of cell types and subsets involved, accurate determination of levels of effector molecules, and the kinetic assessment of these same effector molecules in real time. Advances in Fluorescence Activated Cell Sorting (FACS) allow the assessment of multiple effector molecules, via intracellular antigen detection, in defined cell subsets, via extracellular antigen detection. However, it does not allow the precise and timely assessment of these same factors, especially after re-stimulation of cell samples. Enzyme-Linked ImmunoSorbent Assay ELISA and Cytometric Bead Array (CBA) allow the accurate determination of one or multiple effector molecules, but information on their cellular origin is often sacrificed. Participants will have a chance to explore the advantages and pitfalls of effector molecule detection with the experts during round table and panel discussions

Meeting Chair: Dr Marc Veldhoen, The Babraham Institute, Cambridge, UK

http://www.regonline.co.uk/CMIdiscussion2012