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Fluorescence recovery after photobleaching (FRAP) – from data acquisition to results interpretation

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Fluorescence recovery after photobleaching (FRAP) is widely used to study dynamics of proteins and protein complexes in living cells. In this Masterclass, I will start by giving an overview of the FRAP technique. Then, I will discuss the technical considerations involved in FRAP experiments, common approaches to interpret the results, and available tests to validate the interpretations. Finally, I will demonstrate examples of how FRAP was used to investigate protein dynamics in a range of biological systems.

This talk is part of the "Life Sciences Masterclass" series.

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Average on weekdays: 9029.03333333 kWh Room LR6, Cambridge University Engineering Department, Trumpington Street Cambridge, CB2 1PZ Faculty of Education, 184 Hills Road, Cambridge, CB2 8PQ, 1S3

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