Abstract

In spite of sharing the same DNA all over the body, organisms comprise of different cell types that fulfil various roles due to different RNA levels. The mere identification of these cell types and functions has taken scientists hundreds of years, and is still ongoing. Now with the arrival of single-cell RNA sequencing technologies, it is possible to map RNA quantities in every cell. This makes it possible to identify tens of previously known cell types, and to discover unknown cell types in the same experiment in a very small biological sample. In my presentation I will give an overview of what is the single-cell RNA -sequencing technology, what are its limitations, as well as talk about the application in my own research to study central genes in human early embryos and to identify subpopulations in immune cells in the blood.