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SUMMARY:Fluorogen Switch - Marcel Bruchez\, Carnegie Mellon
DTSTART:20180207T140000Z
DTEND:20180207T150000Z
UID:TALK101164@talks.cam.ac.uk
CONTACT:Vanessa Blake
DESCRIPTION:Fluorogen activating proteins that activate the fluorescence o
 f triarylmethane dyes have been demonstrated as practical tags for both ce
 ll surface and intracellular labelling\, with applications ranging from si
 ngle-molecule imaging to whole-animal optogenetics. The binding of a fluor
 ogenic dye can result in thousands-fold activation\, serving as a binding 
 mediated optical switch\, which activates fluorescence from otherwise dark
  molecules. Synthesis of various fluorescent donors linked to a far-red ex
 citable fluorogen at distances far shorter than the Forster radius of the 
 dyes has established a new family of FRET-based multi excitation fluorogen
 ic dyes\, with tunable excitation and emission properties suitable for use
  with a wide variety of conventional and superresolution microscopy method
 s. Use of environmentally sensitive donor dyes produces targeted and activ
 ated ratiometric fluorescent indicators\, enabling optical physiology at a
 nd beyond the diffraction limit. I will discuss applications of pH sensors
  in living cells for measurement of endolysosomal trafficking and developm
 ent and validation ROS generating and sensor dyes for use in live cells an
 d model organisms.
LOCATION:Lecture Theatre 1\, Department of Chemical Engineering and Biotec
 hnology\, West Cambridge Site
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