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SUMMARY:Single cell seminar: &quot\;Duplex sequencing at genome scale&quot
 \;\; &quot\;Charting the diversification of mammalian cells at whole organ
 ism scale&quot\;  - Rob Osborne (CASM\, Sanger)\; Jonny Griffiths (CRUK CI
 )
DTSTART:20180427T140000Z
DTEND:20180427T150000Z
UID:TALK105208@talks.cam.ac.uk
CONTACT:Lia Chappell
DESCRIPTION:Please come along the monthly campus Single Cell Seminar serie
 s. All are welcome\, including colleagues from Cambridge (though email us 
 so we can book you in as a visitor with security)\n\nThis month we have tw
 o exciting talks\, please join us C302 from 3.00 - 4.00pm THIS FRIDAY (27t
 h April)\n\n1) Rob Osborne (CASM\, Sanger): "Duplex sequencing at genome s
 cale"\n\n2) Jonny Griffiths (CRUK CI): "Charting the diversification of ma
 mmalian cells at whole organism scale”\n\n\nRob’s abstract:\n"High-thr
 oughput sequencing of somatic mutations is required for clinical medicine 
 and for fundamental questions in cancer\, aging and normal development. Cu
 rrent sequencing methods trade off analytical specificity with genome-cove
 rage. Bulk sequencing has low specificity but high genome-coverage. In con
 trast\, sequencing methods incorporating error-correction have high specif
 icity but are inefficient and expensive to apply to whole-genomes. I will 
 describe progress towards an improved duplex sequencing method that provid
 es a simple and automatable route to accurate genome-wide detection of som
 atic mutations."\n\n\nJonny’s abstract: \n"Decision-making is a critical
  component of cellular behaviour\, with errors giving rise to e.g.\, devel
 opmental failures or cancer. Embryonic development is an effective system 
 for studying decision-making\, as it provides a large set of robust\, biol
 ogically relevant\, relatively well-studied decision points. However\, tra
 nscriptomic assays have historically been hamstrung by the extreme spatial
  complexity and molecular heterogeneity of the developing embryo. \n\nWe h
 ave captured 100\,000 single cells for single-cell RNAseq from whole mouse
  embryos during gastrulation and organogenesis\, spanning days 6.5 to 8.5 
 of development\, including embryonic and extraembryonic tissues. Cells wer
 e sampled every six hours\, providing a continuous molecular characterisat
 ion of these processes. We highlight the utility of this rich dataset in t
 hree ways. First\, we reconstruct the development of the three germ layers
  and their lineages. Second\, we identify non-negligible populations of ra
 re cell types such as primordial germ cells. Third\, we dissect one specif
 ic developmental process by considering the contribution of extraembryonic
  visceral endoderm to embryonic endoderm lineages."
LOCATION:C302\, Sulston Building\, Wellcome Genome Campus\, Hinxton. CB10 
 1SA
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