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SUMMARY:Weighing single molecules with light - Philipp Kukura\, Department
  of Chemistry\, Physical and Theoretical Chemistry Laboratory\, University
  of Oxford
DTSTART:20180517T140000Z
DTEND:20180517T150000Z
UID:TALK105766@talks.cam.ac.uk
CONTACT:Patrick Flagmeier
DESCRIPTION:The cellular processes underpinning life are orchestrated by p
 roteins and the interactions they make with themselves and other biomolecu
 les. A range of techniques has been developed to characterise these associ
 ations\, operating from the ensemble all the way to the single molecule le
 vel. Structural and dynamic heterogeneity\, however\, continues to pose a 
 fundamental challenge to existing analytical and structural methodologies\
 , despite being key to protein and drug function. I will present recent de
 velopments demonstrating that interferometric scattering mass spectrometry
  (iSCAMS) can mass-image single biomolecules in solution with simultaneous
  nanometre precision and mass accuracy comparable to native mass spectrome
 try in the gas phase. As a result\, we can resolve oligomeric distribution
 s at high dynamic range\, detect small-molecule binding\, and quantitative
 ly mass-image not only biomolecules composed of amino acids\, but also het
 erogeneous species such as glyco- and lipoproteins. These capabilities ena
 ble us to determine the equilibrium and rate constants and thereby the mol
 ecular mechanisms of processes as diverse as homo- and hetero-oligomeric p
 rotein assembly\, amyloidogenic protein aggregation and actin polymerisati
 on. Our results illustrate how single molecule mass imaging provides unive
 rsally-applicable and spatially-resolved access to the mechanisms and dyna
 mics of protein assemblies\, their interactions and how they form nano- an
 d mesoscopic structures\, one molecule at a time.
LOCATION:Department of Chemistry\, Cambridge\,  Pfizer Lecture Theatre
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