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SUMMARY:Genome Architecture Mapping: discovering 3D genome topology in rar
 e cell types - Ana Pombo\, Berlin Institute for Medical Systems Biology\, 
 Max Delbrueck Center for Molecular Medicine\, Berlin\, Germany\; Humboldt 
 University of Berlin\, Berlin\, Germany
DTSTART:20190131T130000Z
DTEND:20190131T140000Z
UID:TALK110800@talks.cam.ac.uk
CONTACT:Kate Davenport
DESCRIPTION:The folding of chromosomes and the structural organization of 
 the genome impacts human health and disease. Long-range physical contacts 
 between non-coding regulatory regions and their target genes regulate gene
  expression. In dividing cells\, chromatin contacts are established when c
 ells exit mitosis to dissolve upon re-entry into mitosis. We set out to in
 vestigate the functional relevance of chromatin contacts in terminally dif
 ferentiated cells\, such as neurons\, which retain their physiology for ye
 ars in living animals. We applied a novel ligation-free technique to map c
 hromatin contacts genome-wide Genome Architecture Mapping (GAM) which is i
 deally suited to study rare cell types. \n\nGAM extracts spatial informati
 on by sequencing the DNA content from a large collection of randomly orien
 tated\, thin nuclear sections\, before quantifying the frequency of locus 
 co-segregation. By applying GAM to mouse embryonic stem cells\, we had pre
 viously identified specific chromatin contacts enriched for interactions b
 etween active genes and enhancers spanning large genomic distances. We hav
 e now develop faster and more affordable versions of GAM\, which are compa
 tible with their application in specific rare cell types without tissue di
 sruption. As a proof-of principle\, we have used GAM to map chromatin cont
 acts genome-wide in specific neuronal subtypes directly from mouse brain. 
 Our work shows that genome architecture is highly cell-type specific and r
 eflects cell-type specific gene expression patterns at both short and long
  genomic distances.\n
LOCATION:CRUK CI Lecture Theatre
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