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SUMMARY:On the Quaternary Structure of Macromolecular Protein Complexes in
  the Absence of Bulk Solvent - Brandon  T. Ruotolo
DTSTART:20080430T093000Z
DTEND:20080430T103000Z
UID:TALK11089@talks.cam.ac.uk
CONTACT:Giorgio Favrin
DESCRIPTION:Compared to our knowledge of protein secondary and tertiary st
 ructure\, the driving forces behind protein quaternary structure are poorl
 y understood.  Over the past three years\, our group has been using ion mo
 bility separation\, a tool that can separate proteins by virtue of their o
 rientationally-averaged size in the gas phase\, in combination with mass s
 pectrometry to probe the influence of bulk solvent on the stability of pro
 tein quaternary structure.  In our experiments we have observed evidence b
 oth of topologies that match what is known about protein assemblies in sol
 ution as well as discrete structural transitions that are only generated i
 n the absence of bulk solvent.  In this presentation\, the gas-phase quate
 rnary structures and structural transitions of protein assemblies that cov
 er a range of known topologies in solution will be discussed.  This discus
 sion will ultimately focus toward our long-term efforts to integrate ion m
 obility and mass spectrometry data in order to determine the topology and 
 architecture of unknown protein complexes.\n\n
LOCATION:Unilever Lecture Theatre\, Unilever Centre\, Department of Chemis
 try
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