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SUMMARY:Mouse Hox gene enhancers analysed in cell culture\; and some propo
 sals on the significance of Hox gene collinearity - Dr Stephen Gaunt\, Dep
 artment of Zoology\, University of Cambridge
DTSTART:20181031T130000Z
DTEND:20181031T140000Z
UID:TALK112369@talks.cam.ac.uk
CONTACT:Kate Criswell
DESCRIPTION:Mouse Hox gene enhancer analysis has previously been conducted
  in embryos transgenic for Hox/lacZ reporter constructs. Here I show how a
 t least some of these studies can be carried out in cultured HepG2 cells u
 sing Hox/luciferase reporters and a doxycycline-inducible expression syste
 m. In particular\, I show the following: 1) Hoxd11 enhancer is activated b
 y Gdf11/Smad signalling\, 2) Hoxb4\, b8 and a7 enhancers are activated by 
 Cdx1 protein\, and 3) Hoxc8 enhancer is activated by the synergistic actio
 n of Gdf11/Smad signalling and Cdx proteins. Hox enhancer analysis in cell
  culture reduces mouse usage\, requires low cost and skill\, permits rapid
  assessment of putative enhancer binding motifs\, and provides abundant ma
 terial for DNA/transcription factor binding studies.
LOCATION:Part II Lecture Theatre\, Department of Zoology
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