BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Talks.cam//talks.cam.ac.uk//
X-WR-CALNAME:Talks.cam
BEGIN:VEVENT
SUMMARY:Long-term stability of human neurons in an organotypic slice cultu
 re system – a new model to study disease mechanisms of neurological diso
 rders - Henner Koch\, University of Tübingen
DTSTART:20190228T120000Z
DTEND:20190228T130000Z
UID:TALK115789@talks.cam.ac.uk
CONTACT:Dr Romina Vuono
DESCRIPTION:Keeping adult human cortical neurons intact for functional and
  morphological analysis for extended periods of time and for targeted mani
 pulation of human brain cells has been a major goal in neuroscience. Prece
 ding work revealed that human organotypic slice cultures can be maintained
  in human cerebrospinal fluid (hCSF) with intact network function. However
 \, the question to what degree the cellular function remains intact has no
 t been answered in detail. In this study we investigated the relative stab
 ility of adult cortical pyramidal neurons maintained in an organotypic sli
 ce culture for up to 14 days in vitro in hCSF. To quantify the functional 
 constancy we measured basic electrophysiological parameters\, as well as m
 orphological properties of pyramidal neurons in these slices over time. We
  found a robust stability of the physiological parameters of neurons maint
 ained in hCSF. In a second step\, we used a virus approach to detect large
  scale cellular integrity in the slices for post-hoc morphology analysis. 
 The data of this study indicate that adult human cortical neurons can be k
 ept intact in slice cultures and can be investigated in vitro in an enviro
 nment as close as possible to the human brain for extended periods of time
 .
LOCATION:James Fawcett Seminar Room\, van Geest Building
END:VEVENT
END:VCALENDAR