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SUMMARY:A DNA methylation reader complex that enhances gene transcription 
 - Jake Harris\, UCLA
DTSTART:20190107T120000Z
DTEND:20190107T130000Z
UID:TALK116209@talks.cam.ac.uk
CONTACT:david baulcombe
DESCRIPTION:DNA methylation generally functions as a repressive transcript
 ional signal but is also known to  activate gene expression. In either cas
 e\, the downstream factors remain largely unknown. Here\, using comparativ
 e interactomics\, we isolated proteins in Arabidopsis thaliana that associ
 ate with methylated DNA. Two SU(VAR)3-9 homologues\, the transcriptional a
 nti-silencing factor SUVH1\, and SUVH3\, were among the methyl-reader cand
 idates. SUVH1 and SUVH3 bound methylated DNA in vitro\, were associated wi
 th euchromatic methylation in vivo\, and formed acomplex with two DNAJ dom
 ain containing homologues\, DNAJ1 and DNAJ2. Ectopic recruitment of DNAJ1 
 enhanced gene transcription in plants\, yeast and mammals. Thus\, the SUVH
  proteins bind to methylated DNA and recruit the DNAJ proteins to enhance 
 proximal gene expression\, thereby counteracting the repressive effects of
  transposon insertion near genes.
LOCATION:Tom ap Rees\, Department of Plant Sciences
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