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SUMMARY:Ribosome profiling and virus infection - Dr Andrew Firth (Dept of 
 Pathology)
DTSTART:20190220T140000Z
DTEND:20190220T150000Z
UID:TALK116302@talks.cam.ac.uk
CONTACT:46925
DESCRIPTION:Abstract: \nWhile standard RNA-Seq experiments can be used to 
 monitor the abundance of every messenger RNA (mRNA) species in a cell\, th
 is is only an inaccurate proxy for actual levels of protein synthesis\, si
 nce different mRNAs can have strikingly different translation efficiencies
 . Further\, mRNA translation efficiency can vary in response to environmen
 tal and developmental stimuli. In contrast\, Ribosome Profiling (Ribo-Seq)
  provides a direct measurement of protein synthesis. Ribosome profiling re
 lies on the fact that a translating ribosome protects ~30 nucleotides of m
 RNA. An RNA nuclease is used to digest unprotected RNA\, the 30-nt ribosom
 e-protected fragments (RPFs) are purified for deep sequencing\, and the se
 quenced fragments are mapped back to the transcriptome\, to give a global 
 snapshot of protein synthesis in the cell. Ribosome profiling has proven t
 o be increasingly valuable in studies of the translation process\, for exa
 mple\, in the discovery of novel translated open reading frames (ORFs)\, d
 etermination of elongation rates\, and identification of sites of ribosome
  pausing. It also has broad application in the analysis of global protein 
 synthesis and has been exploited in studies of infectious diseases\, cell 
 growth\, differentiation and development\, mitochondrial gene expression\,
  and cell stress. In collaboration with the Brierley lab (http://www.path.
 cam.ac.uk/directory/ian-brierley) we have been applying Ribo-Seq and paral
 lel RNA-seq to a variety of virus species to study the kinetics of virus g
 ene expression\, unusual transcriptional and translational mechanisms empl
 oyed by viruses\, and host responses to virus infection at both the transc
 riptional and translational levels. 
LOCATION:MR4\, Centre for Mathematical Sciences\, Wilberforce Road\, Cambr
 idge
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