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SUMMARY:LMB Seminar Series - Modifications of RNA: their function and role
  in cancer  - Tony Kouzarides\, PhD FRS FMedSci\, Professor of Cancer Biol
 ogy\, Cancer Research UK Gibb Fellow\, Director of the Milner Therapeutics
  Institute\, Member of the Department of Pathology
DTSTART:20190516T093000Z
DTEND:20190516T103000Z
UID:TALK116650@talks.cam.ac.uk
CONTACT:72112
DESCRIPTION:Many modifications are known to exist on tRNA and rRNA but ver
 y few have been detected on mRNA and ncRNA. We assume that this imbalance 
 is due to the lower abundance and complexity of the latter and therefore a
 n issue of detection. We have therefore developed sensitive tools for the 
 detection of modifications on mRNA and ncRNA including\, mass –spec tech
 nology\, antibodies and chemical reactivity assays. We have also defined a
 n atlas of RNA modifying enzymes required for the viability of AML-leukaem
 ia cells using CRISPR approaches. \n\nUsing these tools we are now investi
 gating the biological function\, mechanism of action and cancer connection
  of several RNA modifications. We have shown that the METTL3 enzyme\, that
  modifies m6A\, is necessary for AML-leukaemia\, and that it functions via
  a chromatin based pathway controlling mRNA translation. We have also defi
 ned a new RNA modification\, internal m7G methylation of miRNAs\, mediated
  by the METTL1 enzyme. We show that this modification affects miRNA proces
 sing by disrupting the formation a G-quadrulpex structure. Biologically\, 
 m7G methylation by METTL1 regulates cell migration via. The broader functi
 on of these and other RNA modifications will be discussed.\n
LOCATION:Max Perutz Lecture Theatre\, Medical Research Council (MRC) (MRC 
 Laboratory of Molecular Biol
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