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SUMMARY:Determinants of tissue-specific transcriptional regulation in a tr
 ansplanted\nchromosome - Duncan Odom (Cancer Research UK\, CRI)\n Institut
 e)
DTSTART:20080521T130000Z
DTEND:20080521T140000Z
UID:TALK11807@talks.cam.ac.uk
CONTACT:Danielle Stretch
DESCRIPTION:Sets of conserved transcription factors are responsible for co
 nserved\ntissue-specific transcription\, yet transcription factor binding 
 events\ndiverge rapidly between closely related species (1\,2).  To decoup
 le the\ndistinct molecular mechanisms that direct transcription factor bin
 ding and\ngene expression we investigated tissue-specific transcriptional 
 regulation\nin a mouse containing human chromosome 21 (the Tc1 mouse) (3).
  Gene\nexpression and transcription intiation occurred at similar syntenic
  genes in\nhepatocytes from humans\, wild-type mice\, and Tc1 mice\; howev
 er\, the\ntranscription initiation occurring in other genomic regions was 
 specified by\nspecies-specific genetic sequences.   Characterization of tr
 anscription\nfactor binding in the Tc1 mice revealed that tissue-specific 
 transcriptional\nregulation is directed almost exclusively by species-spec
 ific genetic\nsequences.  High-resolution interrogation of transcription f
 actor binding in\nTc1 mice using deep sequencing established the genetic s
 equences that\nre-direct tissue-specific transcription factor binding in a
 n entirely\nuniform nuclear environment.  Divergent patterns of transcript
 ional\nregulation coded in genetic sequence can thus be transplanted betwe
 en\nspecies to recapitulate conserved transcription in homologous tissues.
LOCATION:MR2\, Centre for Mathematical Sciences\, Wilberforce Road\, Cambr
 idge
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