BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Talks.cam//talks.cam.ac.uk//
X-WR-CALNAME:Talks.cam
BEGIN:VEVENT
SUMMARY:LMB Seminar Series - Structural and mechanistic studies of key com
 ponents in DNA damage signalling and repair - Xiaodong Zhang
DTSTART:20190618T100000Z
DTEND:20190618T110000Z
UID:TALK119005@talks.cam.ac.uk
CONTACT:72112
DESCRIPTION:DNA double-strand breaks (DSB) is one of the most severe types
  of DNA damage and cells have evolved a range of repair pathways including
  homologous recombination to deal with DSB. In eukaryotes\, additional com
 plications are due to the inaccessibility of the damaged sites\, which are
  wrapped in nucleosomes and chromatin.  Unsurprisingly\, a large number of
  proteins and macromolecular complexes\, including many major tumour suppr
 essors such as ATM\, ATR\, BRCA1 and BRCA2\, play central roles in homolog
 ous recombination. ATM (yeast Tel1) and ATR (yeast Mec1) are involved in d
 amage signalling to coordinate the repair events with cell cycle progressi
 on whereas BRCA1 and BRCA2 are directly involved in recruiting and assembl
 y of Rad51 recombinase. A number of chromatin remodellers including the mu
 lti-subunit INO80 complex have also been implicated in this pathway althou
 gh their precise roles are unclear. Apart from these large proteins and co
 mplexes\, a central player is the heterotrimer protein RPA\, which binds t
 o single stranded DNA\, acting as a crucial intermediate in homologous rec
 ombination and replication. Our research focuses on elucidating the struct
 ures and mechanisms of the major macromolecular complexes involved in this
  pathway\, primarily using cryo electron microscopy techniques. In this se
 minar\, I will discuss our recent progress on a number of key components a
 nd our current mechanistic understanding of these proteins and macromolecu
 lar complexes.
LOCATION:Max Perutz Lecture Theatre\, Medical Research Council (MRC) (MRC 
 Laboratory of Molecular Biol
END:VEVENT
END:VCALENDAR