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SUMMARY:Analyses and Diagnostics based on Nanomechanics - Prof. Christoph 
 Gerber\, University of Basel\, Switzerland
DTSTART:20080516T133000Z
DTEND:20080516T143000Z
UID:TALK12096@talks.cam.ac.uk
CONTACT:Dr James Bendall
DESCRIPTION:In recent years we have taken AFM Technology way beyond imagin
 g  exploring  new frontiers in bio analyses  and diagnostics. \nMicro-fabr
 icated silicon cantilevers arrays offer a novel label-free approach where 
 ligand-receptor binding interactions occurring on the sensor generate nano
 mechanical signals like bending or a change in mass that is optically dete
 cted in-situ. We report the detection of multiple unlabelled biomolecules 
 simultaneously down to picomolar concentrations within minutes. Differenti
 al measurements including reference cantilevers on an array of eight senso
 rs enables sequence-specific detection of unlabelled DNA and is suitable t
 o detect specific gene fragments within a complete genome (gene fishing). 
 Expression of  detection of inducible genes and  the  detection of total R
 NA fragments in a unspecific back ground.will be shown.   \nLigand-recepto
 r binding interactions\, such as antigen recognition will be presented. An
 tibody activated cantilevers with  sFv (single chain fragments) which bind
  to the indicator proteins show a significant  improved sensitivity which 
 is comparable with SPR (Surface Plasmon Resonance). \nIn addition  this te
 chnology offers a brought variety  of receptor molecules application such 
 as e.g. membrane protein recognition\, micro-organism detection\, enantiom
 eric separation. \nNew coating procedures\, enlargement of the active surf
 ace area by dendritic molecules as well as improvement of the receptor-can
 tilever chemical bond will be presented. \nThis new findings may lead to a
  novel  individual diagnostic assay  in a combined label-free GENOMICs and
  PROTEOMIC biomarker sensor (COMBIOSENS) \nWe foresee   this novel technol
 ogy  being used as a tool to be applied  in the upcoming field of  system 
 biology  and preventive medicine to evaluate treatment response efficacy f
 or personalized medical diagnostics. 
LOCATION: IRC in Superconductivity Seminar room
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