BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Talks.cam//talks.cam.ac.uk//
X-WR-CALNAME:Talks.cam
BEGIN:VEVENT
SUMMARY:Determination of protein structure and dynamics with integrative 
 approaches - Massimiliano Bonomi\, Structural Bioinformatics Unit\, Instit
 ut Pasteur
DTSTART:20190529T093000Z
DTEND:20190529T103000Z
UID:TALK122752@talks.cam.ac.uk
CONTACT:Gabriella Heller
DESCRIPTION:Cryo-electron microscopy is rapidly emerging as a powerful tec
 hnique to determine the structures of complex macromolecular systems elusi
 ve to other techniques. Since many of these systems are highly dynamical\,
  characterizing also their movements is a crucial step to unravel their bi
 ological functions. In this talk\, I will present an integrative modelling
  approach to simultaneously determine structure and dynamics from cryo-ele
 ctron microscopy density maps. By quantifying the level of noise in the da
 ta and dealing with their ensemble-averaged nature\, this approach enables
  the integration of multiple sources of information to model ensembles of 
 structures and infer their populations. I will illustrate the method with 
 two examples: the characterization of structure and dynamics of the ClpP p
 rotease by a combined cryo-EM and NMR approach and the study of the effect
  of acetylation on microtubule structure and stability.
LOCATION:Department of Chemistry\, Cambridge\, Unilever lecture theatre
END:VEVENT
END:VCALENDAR