BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Talks.cam//talks.cam.ac.uk//
X-WR-CALNAME:Talks.cam
BEGIN:VEVENT
SUMMARY:Proteomic analysis of cell state transitions  - Dr Tony Ly\; Sir H
 enry Dale Fellow\, Wellcome Centre for Cell Biology\, Univeristy of Edinbu
 rgh 
DTSTART:20190612T110000Z
DTEND:20190612T120000Z
UID:TALK124834@talks.cam.ac.uk
CONTACT:Bobbie Claxton
DESCRIPTION:The Ly group focuses on two main areas: 1) the technological d
 evelopment of mass spectrometry-based proteomics to comprehensively measur
 e protein parameters in rare\, transient cell states and 2) the control of
  cell state transitions\, including the quiescence to proliferation switch
  and mitotic entry. Previously\, we developed a method to perform proteomi
 cs of intracellular immunostained subsets\, which we call PRIMMUS (Ly et a
 l.\, eLife 2017). The PRIMMUS approach avoids potential artefacts associat
 ed with inhibitor-based synchronisation and allows for high resolution pur
 ification and analysis of specific\, transiently populated cell cycle stat
 es. In my talk\, I will present recent\, unpublished advances that enable 
 proteome analysis of 2\,000 human lymphoblastoid cells to a depth of ~5\,0
 00 proteins. Additionally\, we have obtained promising preliminary results
  applying PRIMMUS to measure proteins in single cells. Our aim is to apply
  this workflow to explore cell-type specificity in proliferation regulatio
 n by comparing the cell cycle regulated network between human cell types.
LOCATION:Babraham - The Cambridge Building - Petersfield Lecture Theatre
END:VEVENT
END:VCALENDAR