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SUMMARY:Developmental lineage mapping by genomic barcoding in the mouse  -
  Dr Reza Kalor\; Havard Medical School\, Bostom\, Massachusetts\, USA
DTSTART:20190618T110000Z
DTEND:20190618T120000Z
UID:TALK124837@talks.cam.ac.uk
CONTACT:Bobbie Claxton
DESCRIPTION: \n \n\n\nCellular barcoding using nuclease-induced DNA mutati
 ons is an effective approach that is emerging for recording biological inf
 ormation\, including developmental lineages. We introduce the homing CRISP
 R system as an effective means of generating DNA barcodes with a high and 
 scalable diversity. We further describe the implementation of this system 
 in a mouse model with multiple genomically-integrated homing guide RNAs (h
 gRNAs). Each hgRNA can generate hundreds of mutant alleles which combine e
 xponentially to create a large number of barcodes. Activating these hgRNAs
  at conception resulted in developmentally barcoded embryos wherein inform
 ation about each cell's lineage was recorded in its genome. We used these 
 recordings to reconstruct early developmental lineages in the mouse and de
 termine the order of axis specification in its brain. Our results provide 
 an enabling and versatile platform for in vivo barcoding and lineage traci
 ng in a mammalian model system. 
LOCATION:Babraham - The Cambridge Building - Petersfield Lecture Theatre
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