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SUMMARY:A rate-limiting process: T cell activation from a single-cell pers
 pective - Dr Arianne Richard\; Cancer Research UK Cambridge Institute\, Un
 iversity of Cambridge
DTSTART:20190924T140000Z
DTEND:20190924T150000Z
UID:TALK128656@talks.cam.ac.uk
CONTACT:Bobbie Claxton
DESCRIPTION:Cytotoxic T lymphocytes (CTLs) are critical for defence agains
 t intracellular pathogens and cancer. CTL differentiation begins when a na
 ïve CD8+ T cell recognizes a peptide-MHC ligand complex through its T cel
 l receptor (TCR)\, triggering a cascade of signalling events that drive ch
 anges in cell metabolism\, growth and function. The interaction of the TCR
  with a particular peptide-MHC complex is exquisitely sensitive\, such tha
 t single amino acid changes in the presented peptide can dramatically alte
 r the activation of responding T cells. Multiple mechanisms have been prop
 osed to underlie this phenomenon\, but these cannot be distinguished throu
 gh snapshot measurements in bulk cellular populations. It is therefore cri
 tical to measure multidimensional molecular activation events in individua
 l cells over time. To this end\, we employ multiple single-cell techniques
  including single-cell RNA sequencing and mass cytometry to investigate th
 e impact of stimulation strength on naïve T cell activation. Our data sho
 w that the strength of stimulation determines the rate but not the cell-in
 trinsic processes of T cell activation. Such a system could allow for a fi
 nite number of rearranged TCRs and a single set of intracellular machinery
  to appropriately respond to a vast array of foreign stimuli.
LOCATION:Babraham - The Cambridge Building - Kings Hedges Room
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