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SUMMARY:Dissecting context dependent cancer signalling processes using CRI
 SPR-based approaches - Dr Evangelina Petsalaki from EMBL-EBI in Hinxton
DTSTART:20200224T130000Z
DTEND:20200224T140000Z
UID:TALK137833@talks.cam.ac.uk
CONTACT:Anna Toporska
DESCRIPTION:Dysregulation in processes such as growth\, migration and diff
 erentiation in cancer cells is often a result of mutations in proto-oncoge
 nes or oncogenes of the cellular signalling pathway. Cancer cells often be
 come dependent on the activity of such oncogenes\, making these oncogenes 
 potential drug targets. While effective\, pharmacological interventions of
  oncogenes are often limited by adaptive resistance which is often a resul
 t of ‘rewiring’ of the signalling pathways that allows bypass of the d
 rug inhibited. Understanding the principles of the signal rewiring and ide
 ntification of players in this process will have profound implications in 
 improving current therapeutic interventions. \nHere\,  a number of CRISPR-
 based approaches is being utilised to dissect the players that govern sign
 al dysregulation mainly in the context of melanoma. \nDr Evangelia Petsala
 ki will describe a computational framework they have recently devised that
  uses publicly available essentiality screens performed in on ~500 cell li
 nes to interrogate the ‘context’ of a context specific gene.  The tool
  was applied to identify the essentiality of signalling related genes in m
 elanoma\, which provides clues to cell-specific signalling pathways used b
 y melanoma cells with a range of mutational backgrounds and drug response 
 profiles. \nDr Petsalaki will also discuss their recent efforts to combine
  arrayed CRISPR-based genetic perturbation with phospho-proteomic measurem
 ents to identify pathway cross-talks in the context of resistance to MAPK 
 pathway inhibitors in BRAFV600E melanoma. The results from this project wi
 ll contribute to better understanding of the principles of cell signalling
  rewiring in cancer progression and drug resistance. \n
LOCATION:CRUK CI Lecture Theatre (Room 001)
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