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SUMMARY:Injectable therapeutic vascularized micro-tissues engineered using
  sacrificial hydrogels - Dr Ninna Rossen (BRIC-University of Copenhagen)
DTSTART:20200508T140000Z
DTEND:20200508T153000Z
UID:TALK141889@talks.cam.ac.uk
CONTACT:Hilde Hambro
DESCRIPTION:Complex 3D micro-tissues and organoids are becoming widespread
  in drug-screening technologies\, but have been used sparingly for cell th
 erapy as current approaches for producing self-organized cell clusters lac
 k scalability or reproducibility in size and cellular organization. To add
 ress this challenge\, we have engineered complex 3D micro-tissues consisti
 ng of a blood vessel core and a mesenchymal stem cell shell that can act a
 s building blocks in a new vascular network for regenerative medicine. The
  micro-tissues are small enough to be injected\, and the core-shell struct
 ure protects them from the shear stress of injection. The microtissues' in
 jectability enables direct delivery of vascular building blocks to disease
 d areas\, thereby obviating surgery in ischemic tissues with impaired woun
 d healing. We introduced a method of using hydrogels as sacrificial scaffo
 lds and a growth factor regime\, which allows cells to form self-organized
  clusters followed by gentle release\, resulting in highly-reproducible mu
 lticellular structures on a large scale. We demonstrated this strategy for
  endothelial cells and mesenchymal stem cells to self-organize into blood-
 vessel units\, which were injected into mice\, and rapidly formed perfusin
 g vasculature. Moreover\, in a mouse model of peripheral artery disease\, 
 intramuscular injections of blood-vessel units resulted in rapid restorati
 on of vascular perfusion within seven days. As cell therapy transforms int
 o a new class of therapeutic modality\, this simple method – making use 
 of the dynamic nature of hydrogels – could offer high yields of self-org
 anized multicellular aggregates with reproducible sizes and cellular archi
 tectures.
LOCATION:Online
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