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SUMMARY:Professor Erwin Peterman\, Vrije Universiteit Amsterdam: Single-mo
 lecule tracking of transmembrane proteins in the chemosensory cilia of C. 
 elegans - Professor Erwin Peterman\, Vrije Universiteit Amsterdam
DTSTART:20201124T150000Z
DTEND:20201124T160000Z
UID:TALK154312@talks.cam.ac.uk
CONTACT:Francesca van Tartwijk
DESCRIPTION:Abstract:\n\nBeing able to adequately react to one’s environ
 ment is essential for survival and procreation. Cilia are vital for the ce
 ll’s ability to sense its environment and rely on a process called intra
  flagellar transport (IFT) for their development\, maintenance and functio
 n in signal-transduction. As a model\, we study C. elegans chemosensory ci
 lia. Motor proteins transport ciliary components along the polarized micro
 tubule axoneme of the cilium. Among the cargoes of IFT are transmembrane p
 roteins involved in signal transduction. We demonstrate the robust\, exten
 sive and reversible ciliary retraction upon external chemical stimulation.
  To elucidate the dynamics underlying this dramatic shift in protein distr
 ibution\, we performed single-molecule imaging of the transmembrane protei
 n OCR-2 in live C. elegans. Analysis of the single-molecule trajectories s
 hows that\, in dendrite and transition zone\, active transport is the prev
 ailing motility mode of OCR-2. In the proximal and distal segments\, howev
 er\, motility is a much more complex\, location-specific interplay between
  active transport\, normal diffusion and sub diffusion. At the tip\, confi
 nement of the membrane proteins plays an important role. Together\, our da
 ta demonstrates an intricate interplay between modes of transportation tha
 t ensure the proper ciliary distribution of OCR-2. These insights in the d
 ynamics of cellular signal-transduction contributes to a wider understandi
 ng of IFT dynamics and to cilia as chemosensory organelles.\n\nBiography:\
 n\nErwin Peterman is a professor in Physics of Living Systems at the Vrije
  Universiteit Amsterdam. Currently he is also Head of the Physics and Astr
 onomy Department. He studied Molecular Sciences at Wageningen University a
 nd did his PhD in the biophysics of photosynthesis at Vrije Universiteit A
 msterdam. After a postdoc in the lab of W.E. Moerner (UC San Diego\, Stanf
 ord University) he returned to the Vrije Universiteit and has become profe
 ssor there. He is a single-molecule biophysicists who combines the develop
 ment of advanced\, often laser-based\, techniques and their application to
  important biological problems. His instrument development focusses on sin
 gle-molecule fluorescence microscopy\, optical tweezers and acoustic force
  spectroscopy. He is cofounder of LUMICKS b.v. a company that develops sin
 gle-molecule instrumentation and has sold them to labs all over the world.
  In his biological applications\, Peterman focusses on understanding intra
 cellular transport and the role of motor proteins therein and DNA and DNA-
 binding proteins.\n\nZoom: https://us02web.zoom.us/j/85143737777 
LOCATION:Zoom
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