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SUMMARY:Methods for rapid 3D fluorescence microscopy - Dr Reto Fiolka\, Un
 iversity of Texas Southwestern Medical Center
DTSTART:20210302T140000Z
DTEND:20210302T150000Z
UID:TALK156145@talks.cam.ac.uk
CONTACT:David Madden
DESCRIPTION:In fluorescence microscopy\, 3D image data is typically formed
  by acquiring a series of 2D images while changing the focal plane. This p
 rocess is slow as it involves mechanically moving the sample or the object
 ive\, and as such forms a bottle neck for volumetric imaging. Here I prese
 nt two applications of remote focusing that can significantly speed up vol
 umetric imaging\, both in two photon raster scanning microscopy and in lig
 ht-sheet fluorescence microscopy. \n\nI will further present a novel way t
 o form projections of microscopic 3D objects under varying viewing angles.
  This method allows capturing views of a 3D sample at ~hundredfold increas
 ed speed compared to conventional 3D image acquisition. This can be valuab
 le if the object is sufficiently sparse\, and one does not need the full 3
 D information. But I will also show that it is possible to obtain three-di
 mensional reconstructions from a few rapidly acquired projections. \n\nAll
  three methods will be carefully characterized for their optical performan
 ce and examples of rapid biomedical imaging will be given.
LOCATION:via zoom - please contact David Madden for the zoom link
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