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SUMMARY:Probing intracellular physicochemical environments at the nanoscal
 e\, one molecule at a time - Ke Xu\, UC-Berkeley
DTSTART:20210224T160000Z
DTEND:20210224T170000Z
UID:TALK156622@talks.cam.ac.uk
CONTACT:Anne Jacobs
DESCRIPTION:Recent advances in super-resolution microscopy methods based o
 n single-molecule imaging (single-molecule localization microscopy\; SMLM)
  have led to ~10 nm spatial resolution and exciting new biology. We are ad
 vancing beyond the structural (shape) information offered by existing meth
 ods\, to reveal (intracellular) functional parameters\, including chemical
  polarity\, diffusivity\, and reactivity\, with nanoscale resolution and s
 ingle-molecule sensitivity. To this end\, we have been developing new stra
 tegies to perform high-throughput\, multidimensional single-molecule spect
 roscopy in the wide-field. In particular\, with spectrally resolved SMLM\,
  we encoded functional parameters into the emission spectra of single prob
 e molecules\, and so unveiled rich\, nanoscale functional and compositiona
 l heterogeneities in the membranes of live mammalian cells. With single-mo
 lecule displacement/diffusivity mapping (SMdM)\, we mapped out intracellul
 ar diffusivity with unprecedented spatial resolution and fidelity\, and he
 nce discovered that diffusion in the mammalian cytoplasm and nucleus are b
 oth spatially heterogeneous at the nanoscale\, and identified the net char
 ge of the diffuser as a previously overlooked\, key determinant of diffusi
 on rate. By adding rich functional dimensions to the already powerful supe
 r-resolution microscopy\, we thus open up new ways to reveal fascinating l
 ocal heterogeneities in both live cells and chemical systems.
LOCATION:https://zoom.us/j/99827103152?pwd=K0ZGZk9xSzBsSHFRbDRwZTF4dExiQT0
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