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SUMMARY:Chromatin and Gene Transcription in Hypoxia - Prof. Sonia Rocha\; 
 Executive Dean of the Institute of Systems\, Molecular and Integrative Bio
 logy\, University of Liverpool
DTSTART:20210617T113000Z
DTEND:20210617T123000Z
UID:TALK157786@talks.cam.ac.uk
CONTACT:Bobbie Claxton
DESCRIPTION:Oxygen is essential for the life of most multicellular organis
 m and changes in oxygen availability have implications in physiological an
 d pathophysiological conditions. In response to hypoxia (low oxygen availa
 bility) transcriptional changes are induced. The transcriptional response 
 to hypoxia is mediated primarily by the Hypoxia Inducible Factor (HIF) fam
 ily of transcription factors. HIFs are activated in hypoxia via inhibition
  of prolyl hydroxylases (PHDs)\, a group of molecular dioxygenases\, which
  require oxygen for their activity. Whilst the role of HIFs in the transcr
 iptional response to hypoxia is well characterised\, little is known with 
 regards to chromatin structure in low oxygen stress and how this coordinat
 es the transcriptional response. It was recently shown that chromatin is s
 ensitive to oxygen levels\, with hypoxia altering histone methylation at h
 ypoxia responsive genes via inhibition of specific JmjC histone demethylas
 es. To further investigate chromatin structure and its role in hypoxia ind
 uced gene transcription\, we have performed ATAC-seq and RNA-seq in human 
 cancer cell lines in acute and prolonged hypoxia. We find that acute hypox
 ia leads to increased promoter accessibility at a subset of hypoxia induci
 ble genes\, and this mostly precedes changes in mRNA expression\, with fur
 ther changes in chromatin structure after prolonged hypoxia. In addition\,
  ChIP-seq data on histone methylation marks (H3K4me and H3K36me3)\, reveal
 ed similar earlier patterns of genomic distribution. Furthermore\, we diss
 ected the impact of HIF in the observed changes of ATAC-seq analysis. This
  work gives insights into the role of chromatin dynamics in regulating the
  transcriptional response to hypoxia and elucidating the mechanisms behind
  these changes may lead to targeted therapies for hypoxia driven diseases.
 \n\nJoin the webinar live using this link – https://zoom.us/j/9301003263
 4
LOCATION:Online via zoom
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