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SUMMARY:LMB Seminar: Molecular views into cellular functions by in-cell cr
 yo-electron tomography - Dr Julia Mahamid\, Group Leader\, EMBL Heidelberg
 \, Germany
DTSTART:20210420T100000Z
DTEND:20210420T110000Z
UID:TALK158338@talks.cam.ac.uk
CONTACT:Scientific Meetings Co-ordinator
DESCRIPTION:Most structural biology focuses on the structure and function 
 of individual macromolecular complexes\, but falls short of revealing how 
 they come together to give rise to cellular functions. As a consequence\, 
 structural and cell biology have traditionally been separate disciplines a
 nd employed techniques that were well defined within the realm of either o
 ne or the other. Here\, cryo-electron tomography (cryo-ET) provides a uniq
 ue opportunity for obtaining structural information across a wide range of
  spatial scales - from whole cells to individual macromolecules. We develo
 p and employ advanced sample preparation techniques for in-cell cryo-elect
 ron tomography\, including cryo-focused ion beam thinning guided by 3D cor
 relative fluorescence microscopy. Preparations of site-specific ‘electro
 n-transparent windows’ in cellular model systems enable assignment of mo
 lecular structures directly from three-dimensional stills of intact cells 
 and reveal their molecular sociology. Using the genome-reduced human patho
 gen Mycoplasma pneumoniae as a model system\, we further developed the syn
 ergistic application of whole-cell crosslinking mass spectrometry\, cellul
 ar cryo-ET and integrative modelling\, and determine in-cell structures of
  transient\, actively transcribing RNA polymerases coupled to a translatin
 g ribosomes. Recent computational breakthroughs now allow resolving these 
 molecular machines to residue-level and reveal small molecule antibiotics 
 bound to their active cite within the intact pathogen. These methodologies
  unlock an enormous potential for novel discovery enabled by label-free st
 ructural cell biology. 
LOCATION:Webinar -  https://zoom.us/j/97074945822?pwd=NWZvc3VEVWJxWmREVXJh
 aisxTkJ5dz09
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