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SUMMARY:Application of Airy beam light sheet microscopy to examine early n
 eurodevelopmental structures in 3D hiPSC-derived human cortical spheroids 
 - Dr Deep Adhya\, Department of Psychiatry
DTSTART:20210512T150000Z
DTEND:20210512T160000Z
UID:TALK160168@talks.cam.ac.uk
CONTACT:Katharina Zuhlsdorff
DESCRIPTION:The inability to observe relevant biological processes in vivo
  significantly restricts human neurodevelopmental research. Advances in ap
 propriate in vitro model systems\, including patient-specific human brain 
 organoids and human cortical spheroids (hCSs)\, offer a pragmatic solution
  to this issue. In particular\, hCSs are an accessible method for generati
 ng homogenous organoids of dorsal telencephalic fate\, which recapitulate 
 key aspects of human corticogenesis\, including the formation of neural ro
 settes—in vitro correlates of the neural tube. These neurogenic niches g
 ive rise to neural progenitors that subsequently differentiate into neuron
 s. Studies differentiating induced pluripotent stem cells (hiPSCs) in 2D h
 ave linked atypical formation of neural rosettes with neurodevelopmental d
 isorders such as autism spectrum conditions. Thus far\, however\, conventi
 onal methods of tissue preparation in this field limit the ability to imag
 e these structures in three-dimensions within intact hCS or other 3D prepa
 rations. To overcome this limitation\, we have sought to optimise a method
 ological approach to process hCSs to maximise the utility of a novel Airy-
 beam light sheet microscope (ALSM) to acquire high resolution volumetric i
 mages of internal structures within hCS representative of early developmen
 tal time points.
LOCATION:Zoom
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