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SUMMARY:Investigating the machinery of bacterial transmembrane transport -
  Emmanouela Petsolari\, Department of Biochemistry
DTSTART:20210520T120000Z
DTEND:20210520T130000Z
UID:TALK160717@talks.cam.ac.uk
CONTACT:Janet Gibson
DESCRIPTION:One of the determinants of antibiotic resistance in microorgan
 isms is the activity of multidrug efflux pumps. These pumps are chromosoma
 lly encoded in bacterial strains and are conserved indicating their biolog
 ical importance and fitness benefit. Bacterial efflux pumps confer resista
 nce by extruding a wide range of substrates such as antibiotics\, organic 
 pollutants and bacterial metabolites\, to name a few. In Gram-negative bac
 teria\, dynamic tripartite assemblies span the multi-layered cell envelope
  which forms a protective barrier against hostile toxic compounds\, and dr
 ive the efflux of noxious compounds that have managed to enter the cell. I
 n this talk\, I will present recent findings from my PhD research and how 
 the integration of structural biochemistry and biophysical techniques such
  as high resolution cryoEM and cryoET complemented with cellular and funct
 ional assays is being used to establish a profound understanding of the me
 chanisms of efflux pumps. By using a plethora of experimental techniques f
 rom the molecular to the cellular scale\, my main goal is to understand ho
 w these macromolecular structures operate within their functional environm
 ent and how to increase susceptibility of bacterial pathogens to antibioti
 cs.
LOCATION:ONLINE - Email organiser to request link - Details to be sent by 
 email
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