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SUMMARY:Uncovering Mechanisms of Cell-type-specific Gene Expression in Ric
 e  - Dr Leonie Luginbuehl\, Molecular Physiology Group\, Department of Pla
 nt Sciences
DTSTART:20220127T130000Z
DTEND:20220127T140000Z
UID:TALK165079@talks.cam.ac.uk
CONTACT:Kumari Billakurthi
DESCRIPTION:Photosynthetic capacity is regulated by several environmental 
 and endogenous factors\, such as light and cell type. In plant species tha
 t use the ancestral C3 photosynthesis pathway\, carbon fixation primarily 
 takes place in leaf mesophyll cells\, while bundle sheath cells show a red
 uced photosynthetic capacity. An important step in the evolution of the mo
 re efficient C4 photosynthesis pathway is the activation of photosynthesis
  in bundle sheath cells and the partitioning of photosynthetic reactions b
 etween these two cell types. This requires that photosynthetic genes are e
 xpressed in a cell-type-specific manner. While previous efforts to underst
 and cell-type-specific gene expression have focused on C4 species\, essent
 ially nothing is known about the cis-regulatory sequences or transcription
  factors that regulate cell-type-specific gene expression in shoots of C3 
 grasses. Using single-nuclei RNA-sequencing\, we generated a single-cell g
 ene expression atlas of shoots of the C3 crop rice during development in t
 he light. This approach identified 18 cell populations that show both comm
 on and unique transcriptional responses to light. To further understand th
 e regulation of gene expression in rice bundle sheath and mesophyll cells\
 , we characterized genome-wide accessible chromatin regions in these two c
 ell types using ATAC-sequencing. Our analysis revealed hundreds of chromat
 in regions that are differentially accessible between rice mesophyll and b
 undle sheath cells and are associated with the cell-type-preferential expr
 ession of the corresponding genes. Within these accessible chromatin regio
 ns\, we identified a number of cis-elements and transcription factors as c
 andidates for regulating gene expression in mesophyll and bundle sheath ce
 lls. Using these data\, we aim to generate a quantitative gene network mod
 el for the regulation of cell-type-specific gene expression in rice leaves
 .\n\nContact reception@plantsci.cam.ac.uk for a Zoom link prior to a talk 
 if you are not on our mailing list. Due to having to go online\, we are re
 stricting the talks to University of Cambridge and alumni to keep them as 
 informal as possible.
LOCATION:Online
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