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SUMMARY:Identification of Potential CCM Candidates in Chlamydomonas reinha
 rdtii - Dr Indu Santhanagopalan\, Physiological Ecology Group\, Department
  of Plant Sciences
DTSTART:20220217T130000Z
DTEND:20220217T140000Z
UID:TALK165088@talks.cam.ac.uk
CONTACT:Kumari Billakurthi
DESCRIPTION:The Carbon Concentrating Mechanism (CCM) in green algae is a b
 iophysical pump delivering high concentrations of intracellular carbon dio
 xide to enhance the fixation of carbon by CBB cycle. This is achieved with
  a large number of transporters for uptake of carbon dioxide and bicarbona
 te ions\, strategically placed carbonic anhydrases\, and compartmentalizat
 ion of rubisco enzyme\, concentrated in the pyrenoid microcompartment.\n\n
 In this talk\, I shall be describing the identification of potential CCM g
 enes by analysis of expression networks in the green alga Chlamydomonas re
 inhardtii. Screening of knockouts for the identified potential CCM genes l
 ed us to two new candidates with unexpected phenotypes. The two mutants- a
  putative ABC transporter called MRP2\, and a gene encoding a protein with
  EF- hand domain identified\; have an unexpected\, enhanced carbon concent
 ration\, and grow better than wild type under carbon limiting conditions. 
 While the exact role and mechanism of the two genes in CCM remains to be e
 lucidated\, physiological measurements and experiments carried out with th
 e two mutants suggest differences in the inorganic carbon transport mechan
 isms. \n\n\nContact reception@plantsci.cam.ac.uk for a Zoom link prior to 
 a talk if you are not on our mailing list. Due to having to go online\, we
  are restricting the talks to University of Cambridge and alumni to keep t
 hem as informal as possible.
LOCATION:Online
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