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SUMMARY:Deciphering the regulation and regulatory function of oncogenic tr
 anscription factors using time-resolved functional genetics - Johannes Zub
 er\, IMP\, Vienna BioCentre
DTSTART:20220401T120000Z
DTEND:20220401T130000Z
UID:TALK165823@talks.cam.ac.uk
CONTACT:Kate Davenport
DESCRIPTION:One of the most fascinating hallmarks of cancer cells is their
  ability to evade normal differentiation and aberrantly self-renew. To mai
 ntain their aberrant identity\, cancer cells strongly depend on transcript
 ion factors (TFs) and chromatin regulators that normally shape development
 al processes. Pathways and co-factors controlling the dynamic expression\,
  activity\, and turnover of oncogenic TFs can provide pharmacological acce
 ss to these formely “undruggable targets”\, but remain poorly defined 
 for most TFs. At the same time\, a more definitive understanding of their 
 direct regulatory functions will be crucial for drug screening and develop
 ment. To meet these challenges\, our lab has pioneered a simple and scalab
 le approach combining auxin-inducible degradation (AID) and SLAM-seq that 
 enables an unbiased analysis of direct transcriptional functions independe
 nt of mRNA and protein half-lives (Muhar et al.\, Science 2018). In parall
 el\, to decipher pathways controlling the expression and turnover of TFs\,
  we have developed a time-resolved FACS-based CRISPR screening assay that 
 enables the systematic identification of regulatory factors independent of
  essential protein functions (de Almeida & Hinterndorfer et al.\, Nature 2
 021). In first studies\, we have used these new tools to gain insight into
  the regulation and regulatory function of MYC. \n\nHybrid Seminar - for t
 hose external to the CRUK CI please register in advance if attending in pe
 rson.  Thank you.\n\nhttps://www.eventbrite.co.uk/e/cruk-ci-seminar-in-can
 cer-professor-johannes-zuber-imp-vienna-biocentre-tickets-309242601947?utm
 _campaign=post_publish&utm_medium=email&utm_source=eventbrite&utm_content=
 shortLinkNewEmail\n\n\nZoom link: https://zoom.us/j/99749197684?pwd=V2o4VT
 FELzYvdVIrRlR0RTJiRjdjZz09\n
LOCATION:CRUK CI Lecture Theatre
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