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SUMMARY:CADHERIN mediated AMIS localisation - Using mouse embryonic stem c
 ells 3D culture as a model  - Xuan Liang (PDN\, Cambridge University) 
DTSTART:20220131T143000Z
DTEND:20220131T153000Z
UID:TALK168977@talks.cam.ac.uk
CONTACT:Elena Scarpa
DESCRIPTION:ndividual cells within de novo polarising tubes and cavities m
 ust integrate their forming apical domains into a centralised apical membr
 ane initiation site (AMIS). This is necessary to enable organised lumen fo
 rmation within multi-cellular tissue. Despite the well documented importan
 ce of cell division in localising the AMIS\, we have found a division-inde
 pendent mechanism of AMIS localisation that relies instead on CADHERIN-med
 iated cell-cell adhesion. Our study of de novo polarising mouse embryonic 
 stem cells (mESCs) cultured in 3D suggest that cell-cell adhesion directs 
 the localisation of apical proteins such as PAR-6 to a centralised AMIS. U
 nexpectedly\, we also found that mESC cell clusters lacking functional E-C
 ADHERIN were still able to form a lumen-like cavity in the absence of AMIS
  localisation and did so at a later stage of development via a ‘closure
 ’ mechanism\, instead of via hollowing. This work suggests that there ar
 e two\, interrelated mechanisms of apical polarity localisation: cell adhe
 sion and cell division. Alignment of these mechanisms in space allows for 
 redundancy in the system and ensures the development of a coherent epithel
 ial structure within a growing organ. 
LOCATION:Online
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