BEGIN:VCALENDAR VERSION:2.0 PRODID:-//Talks.cam//talks.cam.ac.uk// X-WR-CALNAME:Talks.cam BEGIN:VEVENT SUMMARY:Phosphorylation-mediated signalling – a new era? - Professor Cla ire Eyers\; Department of Biochemistry &\; Systems Biology\, University of Liverpool DTSTART:20220422T123000Z DTEND:20220422T133000Z UID:TALK170522@talks.cam.ac.uk CONTACT:Bobbie Claxton DESCRIPTION:Protein phosphorylation is a ubiquitous post-translational mod ification (PTM) that regulates all aspects of life. To date\, investigatio n of human phosphorylation-mediated cell signalling has focused on canonic al phosphorylation of serine (Ser)\, threonine (Thr) and tyrosine (Tyr) re sidues. However\, mounting evidence indicates that phosphorylation of hist idine also plays a central role in regulating human cell biology. Biochemi cal and phosphoproteomics workflows rely on acidic conditions or elevated temperatures for analysis\, which are incompatible with the analysis of ac id-labile phosphorylation such as histidine. Consequently\, the extent of ‘non-canonical’ phosphorylation in humans and other vertebrates is unk nown. We have developed an Unbiased Phosphopeptide enrichment strategy bas ed on Strong Anion Exchange (SAX) chromatography (UPAX)\, which permits en richment of acid-labile phosphopeptides for characterisation by mass spect rometry. This approach has allowed us to identify extensive and positional phosphorylation patterns on histidine\, arginine\, lysine\, aspartate\, g lutamate and cysteine in human cell extracts. Our data reveals the previou sly unappreciated diversity of protein phosphorylation in human cells\, an d opens up avenues for exploring roles of acid-labile phosphorylation\, an d their interplay with other PTMs\, on human proteins.\n\nClaire E. Eyers is Professor of Biological Mass Spectrometry in the Institute of Systems\, Molecular & Integrative Biology at the University of Liverpool (UoL)\, Di rector of the Centre for Proteome Research (CPR)\, and Associate Pro Vice Chancellor (Research & Impact) for the Faculty of Health and Life Sciences . Having obtained a PhD (2002) in Biochemistry from the University of Dund ee (Prof. Sir P. Cohen)\, she undertook postdoctoral studies at the Univer sity of Colorado\, Boulder (Prof. N. Ahn) and then in the Michael Barber C entre for Mass Spectrometry\, University of Manchester (Prof. S. Gaskell)\ , where she became Acting Director (2009–2013). Her research exploits b iophysical and biochemical methodologies to understand the structure and r elevance of post-translational modifications (PTMs) and their roles in reg ulating cellular signalling in health and disease. She has established exp ertise in the development of mass spectrometric (MS)-based methods\, and e xploits separation technologies\, including ion mobility spectrometry (IMS )\, for the structural investigation of proteins and the effects of PTMs a nd ligand binding. \nProf. Eyers has held an independent American Heart As sociation (AHA) fellowship\, for which she was awarded an AHA postdoctoral prize\, and a Royal Society Dorothy Hodgkin Fellowship (2007-11). She is currently Chair of BBSRC committee D\, Deputy Chair of the BBSRC sLOLA com mittee and formerly trustee and executive committee member of the British Mass Spectrometry Society. She is an active STEM ambassador.\n\n\nClick he re to join live - https://us06web.zoom.us/j/88082921082 LOCATION:Online via zoom END:VEVENT END:VCALENDAR