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SUMMARY:Fast cryo-EM sample preparation: Recent developments and applicati
 ons - David Klebl\, University of Leeds
DTSTART:20220526T090000Z
DTEND:20220526T100000Z
UID:TALK174719@talks.cam.ac.uk
CONTACT:Scientific Meetings Co-ordinator
DESCRIPTION:Please register your interest in advance if you wish to attend
  in person as there will be limited space on the day\, by sending an email
  to lecturet@mrc-lmb.cam.ac.uk\n\nSince the ‘resolution revolution’\, 
 the field of cryo-electron microscopy (cryo-EM) has continued its rapid de
 velopment with new and improved hardware and processing algorithms. Sample
  preparation for cryo-EM is increasingly becoming a bottleneck\, and alter
 natives to the traditional blotting method are needed. We have been develo
 ping methods for fast droplet-based sample preparation\, which allow grid 
 preparation on the timescale of ~10 ms. This has been instrumental to bett
 er understand the interactions between particles and the air-water interfa
 ce and has allowed time-resolved structural studies. As an example\, we ha
 ve solved the first structure of an actomyosin complex in the pre-power st
 roke state by time-resolved cryo-EM. Our current work focuses on reducing 
 the timescale of grid preparation to less than 1 ms\, and on characterisin
 g the interactions between proteins and the air-water interface in more de
 tail
LOCATION:In person in the Sanger Seminar Room (CB2 0QH)
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