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SUMMARY:The B cell receptor and beyond: specialized functions of the BCR i
 n the intestine - Dr Carla Nowosad\; NYU Grossman School of Medicine
DTSTART:20220831T123000Z
DTEND:20220831T133000Z
UID:TALK176471@talks.cam.ac.uk
CONTACT:Bobbie Claxton
DESCRIPTION:B lymphocytes are central to maintaining balanced enteric resp
 onses through production of immunoglobulins (Igs). A large fraction of Igs
  are produced in germinal center (GC) responses\, which form upon immuniza
 tion or infection in the majority of secondary lymphoid organs\, but are c
 onstitutively present in gut-associated lymphoid tissue. However\, gut-ass
 ociated GC responses are poorly understood in terms of their ontogeny and 
 antigenic drivers. Using a multicolor fate-mapping approach to visualize G
 C clonal expansions with a confetti fluorescent allele\, we observed singl
 e-color GCs in the steady state intestine\, suggestive of a strong selecti
 on event. By coupling fate-mapping gnotobiotics and Ig sequencing\, we sho
 wed affinity-driven selection occurring in response to members of the micr
 obiome. We observed that GCs persist even in the absence of microbiota\, a
 lthough their isotype distribution and clonal composition are altered. We 
 identified several prominent public Ig clonotypes in intestinal GCs\, whos
 e presence were tuned by microbial diversity.\n\nClick here to join live -
  https://us06web.zoom.us/j/87391153485
LOCATION:Online via zoom &amp\; Kings Hedges Room 
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