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SUMMARY:Identifying the network of bactericidal interactions between mycob
 acterial respiratory chain complexes to develop fast-acting drugs for tube
 rculosis - Professor Gregory M. Cook\, University of Otago\, New Zealand
DTSTART:20221011T140000Z
DTEND:20221011T150000Z
UID:TALK183218@talks.cam.ac.uk
CONTACT:Hannah Burns
DESCRIPTION:Mycobacterium tuberculosis remains a leading cause of infectio
 us disease morbidity and mortality. Although a curable disease requiring a
  combination of four drugs for six months\, drug-resistant strains require
  long treatment times (>18 months) with limited and often highly toxic dru
 gs. New combination therapies that optimize the synergistic or synthetic l
 ethal interactions between drugs have the potential to drastically reduce 
 treatment times and increase cure rates. However\, the identification of n
 ovel combination therapies is limited by available antibiotics that inhibi
 t only a small number of biological targets. To address this lack in knowl
 edge we have utilized a combination of mycobacterial CRISPR interference (
 CRISPRi) and phenotypic assays of bacterial growth and viability to identi
 fy respiratory complexes in Mycobacterium tuberculosis that when simultane
 ously inhibited result in cell death. The results from this study provide 
 insight into the functional interactions between bioenergetic complexes\, 
 the clinical promise of targeting these processes and the general utility 
 of CRISPRi in designing optimised drug combinations for tuberculosis.
LOCATION:MRC MBU\, Level 7 Lecture Theatre\, The Keith Peters Building\, C
 B2 0XY
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