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SUMMARY:Do you have it or don't you? Walking the line of aDNA pathogen ide
 ntification in low abundance samples - Jess Hider\, Ancient DNA Centre\, M
 cMaster University
DTSTART:20221129T131500Z
DTEND:20221129T140000Z
UID:TALK185315@talks.cam.ac.uk
CONTACT:Taylor Peacock
DESCRIPTION:We analyzed 14 samples from the mummified remains of Sante Bra
 ncorsini\, a 14th century Franciscan friar from central Italy\, with macro
 scopic diagnosis of probable brucellosis.\nShotgun sequencing data from 14
  samples were examined to determine the presence of Brucella DNA. Three of
  the 14 samples contained authentic ancient DNA\, identified as belonging 
 to B. melitensis. A genome (23.81X depth coverage\, 0.98 breadth coverage)
  was recovered from a kidney stone. Nine of the samples contained reads cl
 assified as B. melitensis (7-169)\, but for many of those\, the data quali
 ty was insufficient to withstand our identification and authentication cri
 teria. We identified significant variation in the preservation and abundan
 ce of B. melitensis DNA present across multiple tissues\, with calcified n
 odules yielding the highest number of authenticated reads.  Our results de
 monstrate variation in the preservation and recovery of pathogen DNA acros
 s tissues\, from non-detectable to genome reconstruction\, in an individua
 l with a paleopathological diagnosis of brucellosis. This study highlights
  the importance of sample selection to the reconstruction of infectious di
 sease burden and highlights the importance of a holistic approach to ident
 ifying disease in the past. Further analysis of how sampling impacts aDNA 
 recovery will improve pathogen aDNA recovery and advance our understanding
  of disease in past peoples.\n\n
LOCATION:Seminar Room\, McDonald Institute for Archaeological Research\, D
 owning Site
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