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SUMMARY:Model of inverse bleb growth explains giant vacuole dynamics durin
 g cell mechanoadaptation - Dr Andrea Cairoli\, DAMTP\, University of Cambr
 idge
DTSTART:20230202T123000Z
DTEND:20230202T133000Z
UID:TALK196522@talks.cam.ac.uk
CONTACT:Prof Ray Goldstein
DESCRIPTION:Cells can withstand hostile environmental conditions manifest 
 as large mechanical forces such as pressure gradients and/or shear stresse
 s by dynamically changing their shape. Such conditions are realized in the
  Schlemm's canal of the eye where endothelial cells that cover the inner v
 essel wall are subjected to the hydrodynamic pressure gradients exerted by
  the aqueous humor outflow. These cells form fluid-filled dynamic outpouch
 ings of their basal membrane called giant vacuoles. The inverses of giant 
 vacuoles are reminiscent of cellular blebs\, extracellular cytoplasmic pro
 trusions triggered by local temporary disruption of the contractile actomy
 osin cortex. Inverse blebbing has been first observed experimentally durin
 g sprouting angiogenesis\, but its underlying physical mechanisms are poor
 ly understood. Here\, we hypothesize that giant vacuole formation can be d
 escribed as inverse blebbing and formulate a biophysical model of this pro
 cess. Our model elucidates how cell membrane mechanical properties affect 
 the morphology and dynamics of giant vacuoles and predicts coarsening akin
  to Ostwald ripening between multiple invaginating vacuoles. Our results a
 re in qualitative agreement with observations from the formation of giant 
 vacuoles during perfusion experiments. Our model not only elucidates the b
 iophysical mechanisms driving inverse blebbing and giant vacuole dynamics\
 , but also identifies universal features of the cellular response to press
 ure loads that are relevant to many experimental contexts.
LOCATION:MR15\,  Centre for Mathematical Sciences\, Wilberforce Road\, Cam
 bridge
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