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SUMMARY:Subcellular renovation: shaping of the mitochondrial inner-membran
 e - Professor Luke Chao | Havard Medical School
DTSTART:20230503T140000Z
DTEND:20230503T150000Z
UID:TALK198184@talks.cam.ac.uk
CONTACT:Penny Peck
DESCRIPTION:Cristae membrane state plays a central role in regulating mito
 chondrial function and cellular metabolism. The protein Optic atrophy 1 (O
 pa1) is an important crista remodeler that exists as two forms in the mito
 chondrion\, a membrane-anchored long form (l-Opa1) and a processed short f
 orm (s-Opa1). We previously used an in vitro supported-lipid bilayer recon
 stitution system to show that stoichiometric levels of the processed\, sho
 rt form of Opa1 (s-Opa1) work together with l-Opa1 to mediate efficient an
 d fast membrane pore opening (Ge et al.\, eLife 2020). Our understanding o
 f the mechanisms for how Opa1 form influences cristae shape has remained l
 ess well defined. We performed in situ cryo-electron tomography of cryo-fo
 cused ion beam milled mammalian cells with well-defined Opa1 states to und
 erstand how each form of Opa1 influences cristae architecture. We describe
  differences in cristae geometry\, membrane spacings\, and subcompartment 
 volumes. We find the absence of l-Opa1 results in mitochondria with wider 
 cristae junctions and cells less sensitive to apoptotic stimuli\, implicat
 ing l-Opa1 mediating the cytochrome c release process via the cristae junc
 tion. We discuss the implications Opa1-dependent organelle ultrastructure 
 in organelle function.
LOCATION:MRC MBU\, Level 7 Lecture Theatre\, The Keith Peters Building\, C
 B2 0XY
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