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SUMMARY:Focusing on mitochondria with super-resolution microscopy - Profes
 sor Stefan Jakobs | Max Planck Institute\, Germany
DTSTART:20231115T150000Z
DTEND:20231115T160000Z
UID:TALK198190@talks.cam.ac.uk
CONTACT:Lisa Arnold
DESCRIPTION:Mitochondrial shapes range from small oval fragments to interc
 onnected networks of tubules that are constantly moving\, fusing\, and div
 iding. The innermost mitochondrial aqueous compartment\, the matrix\, is b
 ounded by the highly convoluted inner membrane\, which in turn is surround
 ed by the outer membrane. The inner membrane projects cristae into the mat
 rix. Depending on the cell type and physiological conditions\, the cristae
  can assume a variety of shapes\, ranging from simple tubular to lamellar.
  \nSeveral key players\, including the MICOS (mitochondrial contact site a
 nd cristae organising system) proteins\, have been described to influence 
 mitochondrial architecture. However\, little is known about the distributi
 on of such proteins within these organelles. A major challenge in obtainin
 g a comprehensive view of the sub-mitochondrial localisation of proteins i
 n mitochondria is that these organelles have a diameter close to the diffr
 action limited resolution of light microscopy\, which largely precludes th
 e use of conventional light microscopy to study sub-mitochondrial protein 
 localisations. Using STED super-resolution microscopy and 3D scanning elec
 tron microscopy\, we investigate sub-mitochondrial protein distributions i
 n living and chemically fixed cells. Mic60\, a subunit of the MICOS comple
 x\, and several of its interaction partners are arranged in complex patter
 ns. The data suggest that MICOS is part of an extended multi-protein inter
 action network that scaffolds mitochondria.\n
LOCATION:MRC MBU\, Level 7 Lecture Theatre\, The Keith Peters Building\, C
 B2 0XY
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