BEGIN:VCALENDAR VERSION:2.0 PRODID:-//Talks.cam//talks.cam.ac.uk// X-WR-CALNAME:Talks.cam BEGIN:VEVENT SUMMARY:Seeing memories through the looking glass: hippocampal circuit act ivity in mice performing spatial and working memory tasks - Prof. Simon R. Schultz\, Centre for Neurotechnology\, Imperial College London DTSTART:20230510T093000Z DTEND:20230510T103000Z UID:TALK199156@talks.cam.ac.uk CONTACT:Alyssa Miller DESCRIPTION:We have recently demonstrated the use of an air-lifted behavio ural platform that is compatible with two-photon microscopy to map place f ields\, using the CA1 hippocampal network as a rich and effective memory r eadout (Go et al\, Front Cell Neurosci 15:19\, 2021). This enables us to e xamine the spatiotemporal dynamics of neural activity as memories are laid down\, and as they are later recalled. I will describe how we have employ ed this system to characterise degradation in memory performance in transg enic mice (5xFAD mice\, which over-express mutant human amyloid precursor protein) performing a circular track memory task\, allowing us to quantify effects on neural ensemble activity in both memory recall and encoding. I n further work\, we have used this platform to study changes in network fu nction in 5xFAD mice performing a forced alternation spatial working memor y task. We see effects related to amyloidosis in both degradation of spati al information in the hippocampus\, and in performance on the alternation task. Finally\, I will discuss the pipeline we have been developing to tra ck the effects of neurodegenerative disease in the same animal through beh aviour\, in vivo multiphoton imaging from targeted brain regions\, and fin ally ex vivo whole-brain two-photon tomography registered to the Allen Bra in Atlas. If there is time\, I will also introduce new work on two-photon mesoscopy\, and computational neuroscience work on neural manifold approac hes for analysing large-scale neural datasets. LOCATION:Department of Chemistry\, Unilever Lecture Theatre END:VEVENT END:VCALENDAR