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SUMMARY:LMB Seminar: Single-molecule studies reveal the dynamics of replic
 ation origin licensing - Stephen P. Bell\, MIT\, Dept. of Biology and HHMI
DTSTART:20230703T100000Z
DTEND:20230703T110000Z
UID:TALK199732@talks.cam.ac.uk
CONTACT:Scientific Meetings Co-ordinator
DESCRIPTION:During each cell cycle\, eukaryotic cells must faithfully repl
 icate their genome\, ensuring exactly one full copy is made to maintain ce
 ll ploidy. The major mechanism to ensure once per cell cycle genomic repli
 cation involves the temporal separation of two key replication events: ori
 gin licensing and helicase activation. We have developed an array of singl
 e-molecule biochemical assays to reveal the kinetics and mechanism of thes
 e processes. I will focus on origin licensing\, and discuss the remarkably
  dynamic events required to assemble the head-to-head dimer of the Mcm2 7 
 replicative helicase encircling the double-stranded DNA associated with th
 e licensed origin. In particular\, I will discuss the role of DNA bending 
 and sliding in the early stages of loading\, how studies of the regulation
  of origin licensing have provided insights into the mechanism of Mcm2-7 r
 ing closing\, and evidence that one protein “leap-frogs” another to dr
 ive loading of the two helicases in opposite orientations. Together\, thes
 e studies reveal a mechanism of origin licensing that ensures two helicase
 s are loaded in opposite orientation and the power of single-molecule stud
 ies to uncover the complexities of biological processes
LOCATION:In person in the Max Perutz Lecture Theatre (CB2 0QH) and via Zoo
 m\, link: https://mrc-lmb-cam-ac-uk.zoom.us/j/93130142776?pwd=dEhGKzFGTkw2
 RGI1enhVSGg0Vm1VZz09
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