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SUMMARY:The secret life of sarcomeres: stochastic heterogeneity of sarcome
 res in beating stem-cell derived cardiomyocytes - Daniel Haertter (Georg-A
 ugust-Universität Göttingen)
DTSTART:20230707T110000Z
DTEND:20230707T113000Z
UID:TALK201190@talks.cam.ac.uk
DESCRIPTION:Sarcomeres are the basic contractile units of cardiac muscles.
  We cultured individual hiPSC-derived cardiomyocytes on biomimetic pattern
 ed substrate. We automatically tracked single sarcomere dynamics from high
 -speed confocal recordings with a custom machine-learning tool. While the 
 emergent cell-level motion is very smooth\, we revealed highly stochastic 
 and heterogeneous motion of single sarcomeres. Rigid mechanical constraint
 s force sarcomeres into a tug-of-war like competition driving dynamic hete
 rogeneity. Analysis of a large data set (>1200 cells) indicates that sarco
 mere heterogeneity is not caused by static non-uniformity among sarcomeres
  (e.g.\, strong/weak units)\, but can be primarily attributed to the stoch
 astic and non-linear nature of sarcomere dynamics and thus occurs intrinsi
 cally during cardiomyocyte beating. We show that a simple dynamic model re
 produces crucial experimental findings by assuming non-monotonic force-vel
 ocity relations for single sarcomeres\, as previously predicted for ensemb
 les of motor proteins. This led us to a novel\, active matter perspective 
 on sarcomere motion\, with sarcomeres as interacting non-linear and stocha
 stic agents.
LOCATION:Seminar Room 1\, Newton Institute
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