BEGIN:VCALENDAR VERSION:2.0 PRODID:-//Talks.cam//talks.cam.ac.uk// X-WR-CALNAME:Talks.cam BEGIN:VEVENT SUMMARY:CompuCell3D simulation model for 3D-cells migrating on flat substr ates. - Rita de Almeida (Universidade Federal do Rio Grande do Sul) DTSTART:20230824T100000Z DTEND:20230824T110000Z UID:TALK204397@talks.cam.ac.uk DESCRIPTION:Mesenchymal cell crawling is a critical process in normal deve lopment\, in tissue function\, and in many diseases.Quantitatively predict ive numerical simulations of cell crawling thus have multiple scientific\, medical\, and technological applications\, such as digital twins. We pres ent a \; low-computational-cost approach to simulate mesenchymal three -dimensional (3D) cell crawling\, implemented as a simulation in the Compu Cell3D simulation environment. The Furth equation\, the usual characteriza tion of mean squareddisplacement (MSD) curves for migrating cells\, descri bes a motion in which\, for increasing time intervals\, cell movementtrans itions from a ballistic to a diffusive regime. Experiments have shown that for short time intervals\, cells exhibit anadditional fast diffusive regi me. Our simulations&rsquo\; MSD curves reproduce the three experimentally observed temporal regimes\,with fast diffusion for short time intervals\, slow diffusion for long time intervals\, and intermediate time -interval-b allistic motion.The resulting parameterization of the trajectories for bot h experiments and simulations allows the definition of time- and lengthsca les that translate between computational and laboratory units. Rescaling b y these scales allows direct quantitative comparisonsamong MSD curves and between velocity autocorrelation functions from experiments and simulation s. Although our simulationsreplicate experimentally observed spontaneous s ymmetry breaking\, short-timescale diffusive motion\, and spontaneouscell- motion reorientation\, their computational cost is low\, allowing their us e in multiscale virtual-tissue simulations. We also propose and validate a migratory \; polarization definition as a proxy to predict cell displ acement. Chemotaxis is simulated as an external stimulus to polarization o rientation and not as an external force acting on the cell. Finally\, we p ropose a stochastic model and its anlytical and numeric solution that desc ribes well out findings. Comparisonsbetween experimental and simulated cel l motion support the hypothesis that short-time actomyosin dynamics affect s longer timecell motility. The success of the base cell-migration simulat ion model suggests its future application in more complex situations\,incl uding chemotaxis\, migration through complex 3D matrices\, and collective cell motion. \; LOCATION:Seminar Room 2\, Newton Institute END:VEVENT END:VCALENDAR