BEGIN:VCALENDAR VERSION:2.0 PRODID:-//Talks.cam//talks.cam.ac.uk// X-WR-CALNAME:Talks.cam BEGIN:VEVENT SUMMARY:Dynamics of Aging Biomolecular Condensates - Priya Banerjee (Unive rsity at Buffalo) DTSTART:20231011T103000Z DTEND:20231011T112000Z UID:TALK204889@talks.cam.ac.uk DESCRIPTION:Biomolecular condensates that form via phase separation are co mplex viscoelastic materials whose properties are thought to influence cel lular functions and pathology. In this talk\, I will present our experimen tal and computational results from measurements of sequence-encoded and ag e-dependent material properties of condensates formed by intrinsically dis ordered prion-like low-complexity domains (PLCDs) [1\, 2]. These measureme nts have revealed that nascent PLCD condensates are viscoelastic Maxwell f luids\, and their sequence-specific dynamical moduli are governed by the s trengths of inter-chain interactions. Further\, PLCD condensates can under go physical aging that leads to dynamical arrest on sequence-specific time scales. However\, the aged PLCD condensates are non-fibrillar\, terminally elastic\, Kelvin-Voigt solids. The timescales of fluid-to-solid transitio ns can be controlled by mutations to spacers that weaken the metastability of fluids. Finally\, I will discuss the dynamical properties of aging con densates beyond PLCD systems and biological mechanisms that enhance the me tastabilities of terminally viscous condensates. \;\n \;\nReferenc es:\n\nAlshareedah I\, Moosa MM\, Pham M\, Potoyan DA and Banerjee PR (202 1) Programmable viscoelasticity in protein-RNA condensates with disordered sticker-spacer polypeptides. Nat Commun 12:6620. doi: 10.1038/s41467-021- 26733-7\n\n\nAlshareedah I\, Borcherds WM\, Cohen SR\, Farag M\, Singh A\, Bremer A\, Pappu RV\, Mittag T\, and Banerjee PR (2023) Sequence-encoded grammars determine material properties and physical aging of protein conde nsates. bioRxiv. doi: 10.1101/2023.04.06.535902\n\n \; LOCATION:Seminar Room 1\, Newton Institute END:VEVENT END:VCALENDAR