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SUMMARY:Transcriptional decoding of Developmental Signals - Sarah Bray (Un
 iversity of Cambridge)
DTSTART:20231019T120000Z
DTEND:20231019T130000Z
UID:TALK205171@talks.cam.ac.uk
DESCRIPTION:To make and organize different tissues\, cells decipher inform
 ation from developmental signalling pathways. Transmitting this informatio
 n accurately\, so that cell-surface signals are translated into correct tr
 anscriptional responses\, is of critical importance. For example\,&nbsp\;d
 osage and dynamics of Notch activity are fundamentally important for devel
 opmental decisions and tissue homeostasis and their mis-regulation underli
 es many diseases including cancers. To decipher the temporal\, quantitativ
 e and mechanistic principles that govern how Notch activity is read by tar
 get enhancers in the living animal we image events in real time within liv
 ing fly tissues. We have been addressing two fundamental questions. (i) wh
 at mechanisms and partners are required to transduce Notch signals at targ
 et genes? To investigate we have used live imaging and single particle tra
 cking to measure the recruitment of transcription complexes to a Notch reg
 ulated gene locus in real time\, starting with a Notch off condition and a
 nalysing the changes that take place when signalling is active and subsequ
 ently switched off. (ii) What are the real time dynamics of transcription 
 in cells responding to Notch and how do these relate to developmental deci
 sions being made. To answer this we are using the MS2/MCP and PP7/PCP syst
 ems to measure\, live\, transcription levels and dynamics from endogenous 
 Notch-regulated genes on a cell-by-cell basis to see how they respond to s
 ignals and what the consequences are when we perturb different steps. Inte
 grating the results from these two questions we are learning how enhancers
  decode levels and dynamics of Notch signalling to make key decisions.&nbs
 p\;
LOCATION:Seminar Room 2\, Newton Institute
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