BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Talks.cam//talks.cam.ac.uk//
X-WR-CALNAME:Talks.cam
BEGIN:VEVENT
SUMMARY:Voltage imaging reveals the dynamic electrical signatures of human
  breast cancer cells - Amanda Foust\, Imperial College London
DTSTART:20231006T110000Z
DTEND:20231006T120000Z
UID:TALK205519@talks.cam.ac.uk
CONTACT:Kirsty Shepherd
DESCRIPTION:Dear All\n\nAmanda Foust will give this seminar 'Voltage imagi
 ng reveals the dynamic electrical signatures of human breast cancer cells'
  in West Hub (West 2) at 12pm on 6th October.\n\nhttps://www.imperial.ac.u
 k/people/a.foust\n\n\nAbstract\nCancer cells feature a resting membrane po
 tential (Vm) that is depolarized compared to normal cells\, and express ac
 tive ionic conductances\, which factor directly in their pathophysiologica
 l behavior. Despite similarities to ‘excitable’ tissues\, relatively l
 ittle is known about cancer cell Vm dynamics. Here high-throughput\, cellu
 lar-resolution Vm imaging revealed that Vm fluctuates dynamically in sever
 al breast cancer cell lines compared to non-cancerous MCF-10A cells. We op
 tically characterized Vm fluctuations of hundreds of human triple-negative
  breast cancer MDA-MB-231 cells. Their Vm exhibited spontaneous\, transien
 t hyperpolarizations inhibited by the voltage-gated sodium channel blocker
  tetrodotoxin\, and by calcium-activated potassium channel inhibitors apam
 in and iberiotoxin. The Vm of MCF-10A cells was comparatively static\, but
  fluctuations increased following treatment with transforming growth facto
 r-β1\, a canonical inducer of the epithelial-to-mesenchymal transition. T
 hese data suggest a correlation between cancer processes and Vm dynamics\,
  in addition to previously measured depolarized resting Vm.  Moreover\, ou
 r Vm imaging platform provides a new avenue for cellular-resolution high t
 hroughput characterization of heterogeneous cancer cell electrical phenoty
 pes.
LOCATION:West Hub\, West 2
END:VEVENT
END:VCALENDAR